Simultaneous electroporation and dielectrophoresis in non-electrolytic micro/nano-electroporation
نویسندگان
چکیده
منابع مشابه
Poster presentation SELF CONTROL OF CELL ELECTROPORATION BY DIELECTROPHORESIS TRANSITION
This paper deals with the transition of the dielectric properties of cells as a marker of their permeabilization. A demonstration of the transition between positive and negative DiElectroPhoresis (DEP) as a reaction to electroporation pulses is achieved in a microfluidic device. Electropermeabilization of cell membrane is broadly used in cell biology for electrotransfert of genes and other mole...
متن کاملFeasibility study for cell electroporation detection and separation by means of dielectrophoresis.
Electroporation is a phenomenon during which exposure of a cell to high voltage electric pulses results in a significant increase in its membrane permeability. Aside from the fact that after the electroporation the cell membrane becomes more permeable, the cells' geometrical and electrical properties change considerably. These changes enable use of the force on dielectric particles exposed to n...
متن کاملElectroporation of the Testis
The mature mammalian testis is a marvelous organ that produces numerous sperm cells during its reproductive phase. This biologically significant process consists of three steps: stem cell self-renewal and differentiation, meiosis and genetic recombination, and haploid cell morphogenesis into sperm (Russell et al., 1990). The first step provides a good model for investigating the molecular mecha...
متن کاملElectroporation of Craniofacial Mesenchyme
Electroporation is an efficient method of delivering DNA and other charged macromolecules into tissues at precise time points and in precise locations. For example, electroporation has been used with great success to study neural and retinal development in Xenopus, chicken and mouse (1-10). However, it is important to note that in all of these studies, investigators were not targeting soft tiss...
متن کاملElectroporation-based applications in biotechnology.
Electroporation is already an established technique in several areas of medicine, but many of its biotechnological applications have only started to emerge; we review here some of the most promising. We outline electroporation as a phenomenon and then proceed to applications, first outlining the best established - the use of reversible electroporation for heritable genetic modification of micro...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2018
ISSN: 2045-2322
DOI: 10.1038/s41598-018-20535-6